The cucumber seed oil was obtained by Soxhlet extraction with the seed as the raw material. Soxhlet extraction of oils from cucumber-seed was studied with single factor test, and the yield of cucumber-seed oils was the evaluation index. The influences of extracting solvent, extracting temperature, extracting time, the rate of raw material , and solvent on the yield of cucumber-seed oils were investigated respectively. The optimal conditions of Soxhlet method were obtained: extracting temperature is 68.5 ℃, extracting time is 8 h, the mass concentration of cucumber-seed oil is 0.071 g/mL, optimal extracting solvent is n-hexane. The oil yield reaches 40.38% under the optimal conditions.